SKED scientists Dr. Kobari (Kagoshima Univ.), Dr. Nagai（TUMSAT） and many researchers and students from Kagoshima univ., University of Kyushu, Univ. of Tokyo, Ehime Univ. FRA, and TUMSAT have participated a research cruise on R/T/V Kagoshima-maru. During the cruise, cutting edge oceanographic instruments, such as ocean glider with turbulence sensors, a turbulence profiler with nitrate sensor, a tow-yo microstructure profiler successfully measured the mixing in the Kuroshio origin.